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. 2019 Jul 16;10:1647. doi: 10.3389/fimmu.2019.01647

Figure 5.

Figure 5

IL-2 produced by CD56+ T cells facilitates NK-ADCC response. (A) Representative flow plots of IFN-γ secretion and CD107a expression of purified NK cells and of NK cells from bulk PBMCs with the stimulation of Ab-opsonized P815 cells. (B) IFN-γ secretion and CD107a expression of purified NK cells were lower than that of NK cells from bulk PBMCs (n = 10) during ADCC response. (C) Secretion of IL-2 during ADCC response. PBMCs were stimulated by P815, Ab-opsonized P815 cells or PI (PMA+ionomycin) for 12 h at 37°C. Culture supernatants were collected and IL-2 level was detected by ELISA. Cell pellets were also collected and IL-2 mRNA level in PBMCs was detected by qRT-PCR (n = 10). (D) Representative flow plots of IL-2 and CD107a expressions on CD56− T cells, CD56+ T cells and NK cells with the stimulation of P815 cells only, Ab-opsonized P815 cells or PI. (E) Comparison of the abilities of IL-2 secretion and CD107a expression among CD56− T cells, CD56+ T cells and NK cells with different stimulators (n = 10). (F) Representative flow plots of IL-2 and CD107a expressions on CD56− T cells, CD56+ T cells and NK cells with the HIV-1 elite plasma in the presence or absence of gp120 protein antigen. (G) Comparison of the abilities of IL-2 secretion and CD107a expression among CD56− T cells, CD56+ T cells and NK cells with different stimulators (n = 5). All P-values are two-tailed and significantly different when the value is <0.05.