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. 2019 Jul 16;10:1647. doi: 10.3389/fimmu.2019.01647

Figure 6.

Figure 6

Dysfunction of CD56+ T cells contributes to the impaired NK-ADCC response in chronic HIV subjects. (A) Representative flow plots of lymphocytes in PBMC and CD56+ T depleting lymphocytes sorted by BD FACS Aria III. (B) Comparison of the abilities of CD107a and IFN-γ expression of NK cells from lymphocytes in PBMC and CD56+ T depleting lymphocytes (n = 5). (C) Comparison of CD107a expression of NK cells in the present or absent of anti-IL-2 antibody during the process of ADCC response. ADCC response is triggered by Ab-opsonized P815 cells (n = 10). (D) Purified NK cells were cultured with or without IL-2 antibody and/or CD56+ T cells (transwell insert) (n = 10). Ab-opsonized P815 cells were added to all wells (top and bottom) to trigger the ADCC response. The frequencies of CD107a+ NK cells between groups were compared. (E) Comparison of frequencies of CD56+ T cells in lymphocytes between HIV-1-infected patients (n = 50) and healthy controls (n = 30). (F) Comparison of the abilities of IL-2 secretion from CD56+ T between HIV-1-infected patients (n = 50) and healthy controls (n = 30), with the stimulations of Ab-opsonized P815 cells or P815 cells alone. Data is shown as mean ± SD. All P-values are two-tailed and significantly different when P-value is < 0.05.