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. 2019 Jul 16;10:1623. doi: 10.3389/fimmu.2019.01623

Figure 4.

Figure 4

miR-21 suppresses the protein levels of IRAK4 expression. (A) Miiuy croaker macrophages were transfected with miR-21 (0, 30, and 60 nM) or control mimics (60, 30, and 0 nM), and the protein levels of IRAK4 were determined by Western blot. (B) Macrophages were transfected with miR-21 inhibitors (0, 30, and 60 nM) or control inhibitors (60, 30, and 0 nM), the protein levels of IRAK4 were determined by Western blot. (C) HEK293 cells were cotransfected with IRAK4 expression plasmid, along with miR-21 (0, 30, and 60 nM) or control mimics (60, 30, and 0 nM), and the protein levels of IRAK4 were determined by Western blot. (D) HEK293 cells were cotransfected with IRAK4 expression plasmid, along with the pre-miR-21 plasmid or pcDNA 3.1 vector. At 48 h post-transfection, the protein levels of IRAK4 were determined by Western blot. (E,F) The miiuy croaker macrophages were transfected with miR-21 or control mimics (E) and miR-21 inhibitors or control inhibitors (F) for 48 h and then stimulated with LPS. The expression level of IRAK4 were measured by real-time qPCR and normalized to β-actin. Data are presented as the means ± SE from three independent triplicated experiments.