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. 2019 Jul 16;10:1623. doi: 10.3389/fimmu.2019.01623

Figure 6.

Figure 6

The expression levels of inflammatory cytokines after IRAK4 interference. (A) Miiuy croaker macrophages were transfected with control siRNA (si-Ctrl) or IRAK4 siRNA (si-IRAK4). After 48 h, IRAK4 protein levels were determined by Western blot. (B–D) After 48 h transfected with si-Ctrl or si-IRAK4, macrophages were then stimulated with LPS for 3 h or 6 h. The expression levels IL-1β (B), IL-6 (C), and IL-8 (D) were determined and normalized to β-actin. All data are representative of at least three independent experiments. **p < 0.01 vs. the controls.