Table 1.
PCR primer information in this study.
| Primers | Sequences (5′-3′) |
|---|---|
| REAL-TIME PCR | |
| miR-21-qRT-F | AGCAACAGCAGTCTGTAAG |
| miR-21-qRT-R | TCCAGTTTTTTTTTTTTTTTGCCA |
| 5.8S rRNA-qRT-F | AACTCTTAGCGGTGGATCA |
| 5.8S rRNA-qRT-R | GTTTTTTTTTTTTTTTGCCGAGTG |
| IRAK4-qRT-F | ATCGGCTAAGCGGACATCA |
| IRAK4-qRT-R | TACCTCGCCTCCATCAAGA |
| IL-1β-qRT-F | CATAAGGATGGGGACAACGAG |
| IL-1β-qRT-R | TAGGGGACGGACACAAGGGTA |
| IL-6-qRT-F | GCGGTAAAGGCATGGATAT |
| IL-6-qRT-R | GTTGTAGTTGGAAGGGCAG |
| IL-8-qRT-F | AGCAGCAGAGTCTTCGT |
| IL-8-qRT-R | TCTTCGCAGTGGGAGTT |
| β-actin-qRT-F | GTGATGAAGCCCAGAGCA |
| β-actin-qRT-R | CGACCAGAGGCATACAGG |
| VECTOR CONSTRUCTION | |
| pre-miR-21-F | CCCAAGCTTTGCGTCATGTCTTTGATAGT |
| pre-miR-21-R | CCGGAATTCGCATACAGACAGGTCGTTGT |
| IRAK4-3′UTR-Wt-SacI-F | CGCGAGCTCTTAAAGCACAGGGGTGAAG |
| IRAK4-3′UTR-Wt-XbaI-R | TGCTCTAGACTGCAGCAAATCCAGAGTT |
| IRAK4-3′UTR-Mt-F | AGTGTGCTCAGACGTCACAGTTTGATGGCTTAATATC |
| IRAK4-3′UTR-Mt-R | TGTGACGTCTGAGCACACTGCAGAGTAGTTGTAATACTT |
| Dr IRAK4-3′UTR-Wt-SacI-F | CGCGAGCTCAGGACACCTGGAGGAGCATC |
| Dr IRAK4-3′UTR-Wt-XbaI-R | TGCTCTAGACAGAGCGAGTCAAGCCGAAG |
| Dr IRAK4-3′UTR-Mt-F | GGAGCTGACTTTCGACTGGGGAACCTCAGACT |
| Dr IRAK4-3′UTR-Mt-R | AGTCGAAAGTCAGCTCCATGGTGGGACTCTTT |
| IRAK4-BamHI-F | CGCGGATCCAATAATTCAGTAACTTCCGC |
| IRAK4-XbaI-R | AACTCTGGATTTGCTGCAG |
| GFP-IRAK4-3′UTR-Wt-HindIII-F | CCCAAGCTTGCTAGTTAAAGCACAGGGGTGAAG |
| GFP-IRAK4-3′UTR-Wt-BamHI-R | CGCGGATCCCTGCAGCAAATCCAGAGTT |
| GFP-IRAK4-3′UTR-Mt-F | AGTGTGCTCAGACGTCACAGTTTGATGGCTTAATATC |
| GFP-IRAK4-3′UTR-Mt-R | TGTGACGTCTGAGCACACTGCAGAGTAGTTGTAATACTT |