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| mutations | calculated relative ΔG intraprotein (kcal/mol) | calculated relative ΔG protein–DNA interaction (kcal/mol) |
|---|---|---|
| wild type | 0 | 0 |
| I85N | 1.9 | –0.1 |
| W116R | 10.0 | –0.2 |
| N118K | 1.8 | –2.7 |
| R121C | 0.7 | 2.4 |
| R121H | 4.1 | 4.0 |
| S125L | 1.0 | 3.9 |
