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(A–E) Cytotoxic effect of β-CD:EV inclusion complex on Caco-2 cells as displayed by the RTCA DP instrument. (A) Cells were seeded overnight to reach the log phase, then incubated with media only, (B) β-CD only, (C) β-CD:EV inclusion complex (contains 3.26 μM EV), (D) β-CD:EV inclusion complex (contains 6.52 μM EV), or (E) β-CD:EV inclusion complex (contains 13.04 μM EV). The IC50 value of β-CD:EV inclusion complex was found to be 17.71 ± 4.45 μM after 96 h incubation time.
