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. 2019 Aug;25(8):1563–1566. doi: 10.3201/eid2508.190281

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This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Evaluation of the effects of suboptimal storage of VSVΔG/LASVGPC in guinea pigs. A) Survival rates. B) Percentage weight loss. Values >100% indicate weight gain. C) Body temperatures. D) Viral titers. For A, B, and C, n = 6; for D, n = 3. Survival analysis was conducted using a log-rank Mantel-cox test. Viral loads in tissues were compared with VSVΔG/ANVDVGPC controls using a 2-way analysis of variance. *p<0.0001; †p = 0.002. Error bars indicate SEM.FT, freeze–thaw; RT, room temperature; TCID₅₀, 50% tissue culture infectious dose; VSVΔG/ANDVGPC, vesicular stomatitis virus–based Andes virus vaccine; VSVΔG/LASVGPC, vesicular stomatitis virus–based Lassa virus vaccine.