Figure 3.

Simultaneous deletion of Tsc1 and Rheb1 prevents mTORC1 epileptogenesis and mTORC1 activation. (A) Cortical protein levels of both TSC1 and RHEB1 are significantly lower in Tsc1‐Rheb1‐Cre⁺ mice compared to Tsc1‐Rheb1‐Cre^– mice. Levels of pS6^Ser240/244 in Tsc1‐Rheb1‐Cre⁺ mice are comparable to Tsc1‐Rheb1‐Cre^– mice and rapamycin‐treated (10 mg/kg) Tsc1‐Cre⁺ mice. (B) Within the same mice, hippocampal samples show significantly lower TSC1 and RHEB1 levels. Levels of pS6^Ser240/244 in Tsc1‐Rheb1‐Cre⁺ mice are comparable to Tsc1‐Rheb1‐Cre^– mice and rapamycin‐treated (10 mg/kg) Tsc1‐Cre⁺ mice. All mice were sacrificed on day 12. Western blot data is presented as means with error bars representing SEM. Sample sizes are indicated in the figures with n the number of mice used. An unpaired t‐test was performed to compare protein levels of TSC1 and RHEB1. A one‐way ANOVA was used for statistical analysis with a Dunnett’s test as post hoc test for pS6^Ser240/244 levels. Tsc1‐RHEB‐Cre^– mice were used as control group. **P < 0.01, ***P < 0.001, ****P < 0.0001.