Figure 5.

TSC1‐mediated epilepsy induces changes in the mTORC1‐pathway that are only partially reversed by rapamycin. (A) Proteins within the mTORC1 pathway that were selected for the pathscan phosphorylation analyses are depicted. The significantly changed proteins are circled in yellow and indicated with a star. (B) Pathscan results of the cortical samples where pS6^Ser235/236 and pRSK1^Ser380 are already significantly higher on day 8. (C) Pathscan results of hippocampal tissue of Tsc1‐Cre⁺ mice sacrificed on day 8, day 13, and day 13 after treatment with 5 mg/kg of rapamycin that started on day 4 after initiating gene deletion. pS6^Ser235/236 levels are significantly higher on day 8 and rescued by rapamycin treatment. Pathscan data are presented as means and error bars represent SEM. Sample sizes are indicated in the figures with n the number of mice used. A one‐way ANOVA was used for statistical analysis with a Dunnett’s test as post hoc test. Tsc1‐Cre^– mice were used as control group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.