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. 2019 Jun 14;6(7):1151–1164. doi: 10.1002/acn3.783

Figure 5.

Figure 5

Immunohistochemistry reveals CD8+ T cells in direct contact with MPs in other MS patients. FFPE fixed tissue sections of brain lesions of patients MS11 perivascular (A–D), MS11 parenchymal (E–H), MS10 parenchymal (I–L), and MS25 perivascular (M–P) were stained for the markers CD163 (turquois), CD8 (magenta), CD11b (white), and nuclei (DAPI, dark blue). We show the four‐color overlay (A, E, I, and M) and single color staining for each of the antigens CD163 (B, F, J, and N), CD8 (C, G, K, and O) and CD11b (D, H, L, and P). Arrows indicate contact between a CD8+ T cell with flattened surface and CD163+ cell. Note the weak expression of CD11b on CD163+ cells (D, H, L, and P), but strong CD11b expression on CD163− cells (L and P). Scale bars 10 μm.