Skip to main content
. 2019 Jun 20;10(1):144–166. doi: 10.1080/19491034.2019.1629795

Figure 8.

Figure 8.

Arabidopsis triple wip mutant phenotype rescued with MLKS2.

DAPI stained representative images of a) leaf and b) root nuclei from three Arabidopsis genotypes: Arabidopsis thaliana WT (Col0); (Columbia WT strain), wip123 (AtWIP-type KASH triple mutant where wip123 refers to genotype wip1-1, wip2-1, wip3-1) [83], or wip123 plus GFP-MLKS2 (WIP triple mutant transformed with GFP-MLKS2). (c, d) Nuclear circularity index summaries for these same tissues and genotypes are shown below each tissue/genotype combination, where 1 is a perfectly round nucleus and values lower than 1 are a measure of nuclear elongation. Arabidopsis WIP triple mutant nuclei are significantly more rounded (leaf CI = 0.81 ± 0.03; root CI = 0.72 ± 0.02) than WT (leaf CI = 0.56 ± 0.03; root CI = 0.39 ± 0.02) whereas GFP-MLKS2 complemented nuclei are similar to WT (leaf CI = 0.52 ± 0.03, root CI = 0.32 ± 0.02); p > 0.05 = ns, p < 0.0001 = ****.