Figure 9.

Summary diagrams and models of ZmMLKS2.

Summary diagram illustrates how MLKS2 may interact with F-actin to produce the genetic and heterologous expression phenotypes reported in this study. a) MLKS2 is presumed to be arranged with an ARM domain-containing alpha solenoid structure in the cytoplasm, where it interacts with F-actin directly (left half) or indirectly through a hypothetical connector depicted by the boxed question mark (right half). b) The mks2-2 mutant has lost the ability to bind or contribute to the recruitment of F-actin. Not depicted here are data from MLKS2 in vegetative (leaf, root) organs. Results from kymograph analysis of GFP-MLKS2 expressed in N. benthamiana are summarized for experiments that showed c) anchored nuclei in cells expressing full length MLKS2 or MLKS2ΔKASH, or d) mobile nuclei in cells expressing a control NE marker (lbr) or MLKSΔARM. In these diagrams, evidence for interaction with ZmSUN2 co-expression is indicated (red) on the basis of FRAP assays or depicted from presumed interactions with tobacco SUN, NbSUN (grey). In these diagrams (c,d), the MLKS2 interaction with F-actin is depicted as direct for convenience, but the alternative indirect mode (A, right half) of MLKS2 interaction with F-actin remains a formal possibility.