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. 2019 Jun 14;47(13):7105–7117. doi: 10.1093/nar/gkz498

Figure 1.

Figure 1.

RRE constructs used in NMR studies. (A) Three-way junction (RREII) and stem IIB (RREIIB) constructs used in this study. The Rev primary and secondary binding sites are indicated using filled and dashed orange circles, respectively. Base pairs with and without detectable H-bonds are indicated using solid and dashed lines, respectively. Residues showing differences in chemical shifts between RREII and RREIIB or that have broadened resonances indicative of micro-to-millisecond exchange are highlighted using green circles and colored red on RREIIB, respectively. (B) Comparison of aromatic 2D 1H,13C-HSQC of RREIIB (black) with RREII (green) and RREIIB plus Mg2+ (red). The sample conditions were 1.0–1.2 mM RREII or RREIIB in 15 mM sodium phosphate, 25 mM NaCl, 0.1 mM EDTA, pH 6.4 with or without 3 mM MgCl2. Resonances exhibiting line-broadening are labeled in red and those with ambiguous assignments denoted using an asterisk.