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. 2019 Jun 14;47(13):7105–7117. doi: 10.1093/nar/gkz498

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Off-resonance R_1ρ NMR measurements in RREIIB. (A) Secondary structure of RREIIB. Nucleotides with no RD, ES1 and/or ES2-specific RD are colored black, green and blue, respectively. Nucleotides for which RD could not be measured are in gray. Syn base is indicated with the red box. (B) Comparison of off-resonance R_1ρ profiles in the presence and absence of 3 mM Mg²⁺. Different power levels (ω2π⁻¹) are color-coded. Solid lines represent the global fits to the RD data. Error bars represent experimental uncertainty based on Monte Carlo analysis of monoexponential decay curves and the signal noise. (C) Representative examples of off-resonance R_1ρ profiles showing resonances with three-state RD and absence of RD. The sample conditions were 1.0–1.2 mM RREIIB in 15 mM sodium phosphate, 25 mM NaCl, 0.1 mM EDTA, pH 6.4 with or without 3 mM MgCl₂.