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RA-induced ESCs early differentiation is blocked in EN-KO cells. (A and B) Volcano plot (A) and heatmap (B) depicting gene expression changes in WT and EN-KO ESCs treated with RA (fold-change ⩾ 2, and P < 0.05, as determined by DESeq2). (C and D) GO-BP and KEGG pathway analyses of indicated differentially expressed genes (top 10). (E) RNA-seq results shown the Heatmap of pluripotency and differentiation genes.
