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Alkylation with AP-site-containing DNA and T-T/U-U mismatch-containing DNA/RNA by acridine-VQ-SPh precursor (3-SPh). The precursor (50 μM) was incubated with target DNA or RNA duplex (2.5 μM) at 37°C in 50 mM MES buffer (pH 7.0) containing 100 mM NaCl. (A) The sequences of the target DNA or RNA. (B) Time course of the reaction yields for AP-site. (C) Time course of the reaction yields for T-T (circle) and U-U (square).
