Figure 6.

Alkylation reaction of VQ-SPhX with G-4 DNA. The precursor ligand (50 μM) was incubated with G-4 DNA folded in K⁺ (2.5 μM) at 37°C in buffer solution. G-4 potassium buffer (K⁺): 100 mM KCl, 10 mM K₂HPO₄/ KH₂PO₄ (pH 7.0), 1 mM K₂EDTA. Time course of alkylation of VQ-SPhX with electron-donating effect (left) and with electron-withdrawing effect (right)