Figure 10.

S. pneumoniae MgtA protein that is associated with the yybP-ykoY riboswitch rescues Mn-sensitivity in B. subtilis. Sensitivity of Mn²⁺ was monitored in B. subtilis WT and ΔmntR cells harboring the mgtA RNA aptamer sequence or MgtA in LB broth containing 0 (darker bars) or 50 μM (lighter bars) Mn overtime. Isopropyl β-D-thiogalactopyranoside (1mM) was added to induce MgtA protein synthesis. (A) 2-h cell density. (B) Total cell associated Mn and Ca determined by IPC-MS after 2.5 h growth. (C) Disk diffusion assay. The mean of at least three independent cultures ± SEM are shown for each experiment. *P-value ≤ 0.05; **P-value ≤ 0.01; ***P-value ≤ 0.001; ns, no significance.