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. 2019 Jun 5;47(13):6885–6899. doi: 10.1093/nar/gkz494

Figure 7.

Figure 7.

Mn2+ activates in vitro transcription of mgtA better than Ca. 32P labeled products produced in presence of Mn (A) and Ca (B) (in a background of 250 μM Mg2+) were separated by PAGE. Termination (T) products reflect terminator formation. Read-through (RT) products form as a result of metal-binding and stabilizing the aptamer thereby preventing formation of the terminator. (C) Quantification of in vitro transcription termination assays, with the continuous lines fit to a Hill model of activation (see text for details).