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. 2019 Jun 5;47(13):6885–6899. doi: 10.1093/nar/gkz494

Figure 9.

Figure 9.

The S. pneumoniae mgtA riboswitch senses and responds specifically to intracellular Mn in B. subtilis. Exponentially B. subtilis cells growing in LB treated with indicated metal for 2 h, then harvested. (A) Relative transcription of PmgtA-lacZ fusion in ΔmntR cells grown with increasing concentrations of Mn. (B) β-Galactosidase activity in ΔmntR carrying various promoter-lacZ fusions in response to increasing concentrations of Mn. (C) β-galactosidase activity in response to various metal cations. (D) β-Galactosidase activity in WT cells containing WT-mgtA leader sequence in response to Mn and Ca. (E) β-galactosidase activity in ΔmntR carrying Pr_full (WT) and mutant forms the mgtA riboswitch in response to various metal cations. The mean of at least three independent cultures ± SEM are shown. Pr_Full, promoter plus intact 5′ UTR (185 nt; see Supplementary Figure S1); Pr_Apt, promoter plus aptamer (nucleotides 1–124); and Pr_Term, promoter plus terminator hairpin (nucleotides 101–185).