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Thermodynamic parameters of cation interactions with the mgtA RNA aptamer
| Parametera | Mn2+ | Ca2+ |
|---|---|---|
| K d (μM) | 54 ± 26 | 1.72 ± 0.03 |
| n | 1.0 ± 0.0 | 1.07 ± 0.0 |
| ΔH (kcal/mol) | 1.1 ± 0.1 | –3.6 ± 0.1 |
| TΔS (kcal/mol) | 7.0 ± 0.2 | 4.3 ± 0.1 |
| ΔG (kcal/mol) | –5.9 ± 0.02 | –7.9 ± 0.2 |
aMean and standard deviations are obtained from duplicated independent experiments. All experiments were performed in a background of 3 mM MgCl2 at 25°C. Data reported here are calculated from a one-site random binding model.
