Fig 1.
General layout of bacterial growth plates (Top Left). L. monocytogenes (Top Right) S. aureus (Bottom Left) and E. coli (Top Right) growth following various cellular disruption techniques including Lyse-It (A), vortex (B), sonication (C), and 60ºC conventional heating (D). L. monocytogenes growth is inactivated following microwave irradiation of 30% power for 60 seconds or with conventional heating for over 1 minute. S. aureus growth is inactivated following microwave irradiation of 30% power for 60 seconds or with conventional heating for over 1 minute. E. coli is inactivated following 30% power for 30 s seconds and over 4 minutes of conventional heating. In all cases, 15 minutes of vortex or sonication does not result in any significant growth inhibition for L. monocytogenes, V. cholerae, S. aureus, or E. coli. Pre = 108 CFU/mL suspension without further processing.