Skip to main content
. 2019 Jul 23;14(7):e0220102. doi: 10.1371/journal.pone.0220102

Fig 4.

Fig 4

qPCR cycle number for various concentrations of V. cholerae (Left) and L. monocytogenes (Right) in solution as compared to extracted off of 903 filter paper post 5 minutes vortex (A, B), 5 minutes sonication (C, D), or microwave irradiation (E, F). (A, C, E) A lower cycle number is obtained when V. cholerae is in solution as compared to releasing cells from filter using vortex, sonication, or 30% power, 60 second microwave irradiation. (B, D, F) When the filter is first rehydrated in water and then vortexed, sonicated, or microwaved at 50% power, 60 seconds, more extraction of L. monocytogenes is detected through qPCR. FP (Filter Paper) = 903 Protein Saver Card.