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. 2019 Jul 23;14(7):e0220102. doi: 10.1371/journal.pone.0220102

Fig 5. qPCR of L. monocytogenes detected from ground spinach with and without Lyse-It and no filtration.

Fig 5

(A) Cycle number comparisons of three incubation times of extraction of L. monocytogenes without Lyse-It. (B) Cycle number comparisons of three incubation times with extraction of L. monocytogenes by Lyse-It. In both (A) and (B), there is no statistical difference in cycle number as incubation time increases. Therefore, cycle numbers were averaged across all incubation times to create (C). In terms of L. monocytogenes extraction and detection through qPCR, there is no statistical difference in cycle number with or without the use of gold Lyse-It. Note: No Lyse-It means simply heating in a microwave cavity.