Fig 6. qPCR of L. monocytogenes detected from ground spinach with and without Lyse-It after filtering the product post lysing.

(A) Cycle number comparisons of three incubation times of extraction of L. monocytogenes without Lyse-It. (B) Cycle number comparisons of three incubation times with extraction of L. monocytogenes by Lyse-It. (C) Average of cycle numbers for the three incubation times. Statistical differences are seen in both the microwave power used for L. monocytogenes extraction as well as for no Lyse-It versus Lyse-It. Note: No Lyse-It means simply heating in a microwave cavity.