Fig 9. Recruitment of CMI1 by ICR1 to MTs depends on Ca²⁺ binding and intact hydrophobic pocket of CMI1 and ICR1 W338.

(A-I) CMI1, but not Ca²⁺ nonbinding cmi1D85N and hydrophobic pocket cmi1L59A, mutants is recruited to MTs by ICR1. (J-L) icr1W338A is associated with MTs but does not recruit CMI1. Each panel is as per legends. Bar, 20 μm for all panels. CMI1, Ca²⁺-dependent modulator of ICR1; ICR, interactor of constitutively active ROP; MT, microtubule; O/L-overlay of mCherry and GFP signals.