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. 2019 Jul 11;17(7):e3000085. doi: 10.1371/journal.pbio.3000085

Table 1. CaCl2 and EDTA composition in the CD spectroscopy buffers.

Buffer name 2 mM CaCl2 *200 μM Ca2+ free *2 μM Ca2+ free *200 nM Ca2+ free *20 nM Ca2+ free *2 nM Ca2+ free 1 mM EDTA
CaCl2 2 mM 1,199.9 μM 998.8 μM 969.5 μM 759.7 μM 240.2 μM -
EDTA - 1 mM 1 mM 1 mM 1 mM 1 mM 1 mM

*Calculations were made using the WEBMAXC EXTENDED server (http://web.stanford.edu/~cpatton/maxc.html).

Abbreviation: CD, circular dichroism.