Table 1. CaCl2 and EDTA composition in the CD spectroscopy buffers.
Buffer name | 2 mM CaCl2 | *200 μM Ca2+ free | *2 μM Ca2+ free | *200 nM Ca2+ free | *20 nM Ca2+ free | *2 nM Ca2+ free | 1 mM EDTA |
---|---|---|---|---|---|---|---|
CaCl2 | 2 mM | 1,199.9 μM | 998.8 μM | 969.5 μM | 759.7 μM | 240.2 μM | - |
EDTA | - | 1 mM | 1 mM | 1 mM | 1 mM | 1 mM | 1 mM |
*Calculations were made using the WEBMAXC EXTENDED server (http://web.stanford.edu/~cpatton/maxc.html).
Abbreviation: CD, circular dichroism.