Fig 8. Dextran-based competitive binding assay to determine relative affinity of MhPA14 for sugars.

The progressive removal of GFP_MhPA14 from dextran-based resin–using a series of monosaccharide hexoses (top) and disaccharides (bottom)–is shown. The presence of released protein was measured using absorbance at 280 nm, normalized to the maximum released protein value (Bmax). Data points were done in triplicate, with standard deviation shown via error bars. Data were fit with non-linear regression.