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. 2019 Jul 19;12:5849–5860. doi: 10.2147/OTT.S210363

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© 2019 Qiao et al.

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hsa_circ_0003998 expression features. (A) Electrophoresis of qRT-PCR products. Lane M is DL2000 Marker, lanes 1–5 are products. (B) Validation of splicing junction site through sequencing of qRT-PCR products. (C) High levels of hsa_circ_0003998 in HCC tissues (n=200, P<0.001). (D) High levels of hsa_circ_0003998 in HCC cell lines. The △Ct value was determined by subtracting the Ct value of GAPDH from the Ct value of hsa_circ_0003998. Larger ΔCt value indicated lower level. **P<0.001 represented the statistical differences.