Table 2. PCR primers.
Primer sequence (5'-3') | |
---|---|
Forward |
F(NA45);TGTCACCATGGAAGAAACAGAAGGGAATAAACAA F(063228, 081661);TTTGGCCATGGATGAAAACTAAATACACAGC |
Reverse |
R(NA45);TGTCAGGATCCTTGAAGAAAGAGAAAAGATTG R(063228); TTTGGGGATCCTTGA AGA AAGATAAGA AACCA R(081661); TTTGGGGATCCTTGAAGAAAGAGAAGATACTG |
Restriction enzyme sites (underlined) for NcoI and BamH1 were included in the forward primer and reverse primers.