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. 2019 Jul 23;14(7):e0219817. doi: 10.1371/journal.pone.0219817

Table 2. PCR primers.

Primer sequence (5'-3')
Forward F(NA45);TGTCACCATGGAAGAAACAGAAGGGAATAAACAA
F(063228, 081661);TTTGGCCATGGATGAAAACTAAATACACAGC
Reverse R(NA45);TGTCAGGATCCTTGAAGAAAGAGAAAAGATTG
R(063228); TTTGGGGATCCTTGA AGA AAGATAAGA AACCA
R(081661); TTTGGGGATCCTTGAAGAAAGAGAAGATACTG

Restriction enzyme sites (underlined) for NcoI and BamH1 were included in the forward primer and reverse primers.