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The Eu-labeled antigen (ZIKV NS1), Alexa-Fluor-labeled protein L and the serum sample are mixed together in a 384-well microplate. The former binds to the light chains of the antibodies, which if ZIKV-NS1-specific also bind the europium-labeled NS1 antigen. The mixture is exposed to UV light and the close proximity allows the donor and acceptor to elicit the FRET signal, which is measured.
