Figure 4. The SMP domain of E-Syt1 alone does not cluster liposomes but transfers lipids between protein-tethered liposomes.

a, Domain structures of E-Syt1 constructs (left) and schematic representation of SMP-mediated lipid transfer between liposomes tethered by the C2ABCDE of E-Syt1 in the presence of Ca²⁺ (right). b, Dot plots showing liposomes aggregation (or the lack thereof), due to tethering of donor and acceptor liposomes in the presence of various E-Syt1 constructs and 100 μM Ca²⁺ at RT, as assessed by the increase in turbidity (OD₄₀₅) levels after incubation for 10 min (arrow in Supplementary Fig. 5b). c, Dot plots showing lipid transfer between donor and acceptor liposomes in the presence of E-Syt1 constructs and 100 μM Ca²⁺ at RT, as assessed by the increase in NBD-PE fluorescence at the end of the incubation (arrow in Supplementary Fig. 5c). Mean and standard deviations (error bars) are shown (n=3 independent experiments).