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. 2019 Jul 16;10(1):282–291. doi: 10.1080/21655979.2019.1632669

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Effects of EGCG on TGF-β1-induced epithelial-to-mesenchymal transition (EMT) in vitro. 8505C cells were pretreated for 1 h with EGCG, followed by incubation with TGF-β1 for 48 h. (a) Expression of EMT markers in TGF-β1-stimulated 8505C cells was detected by western blot assay. (b) Immunofluorescence double staining for E-cadherin (red) and vimentin (red) in TGF-β1-stimulated 8505C after treatment of EGCG. Cells was counterstained with Hoechst 33342 (blue). Magnifications ×200; (c) Expression of the E-cadherin transcription repressors in TGF-β1-stimulated 8505C cells was detected by western blot assay. The data are representative of three similar experiments and quantified as mean values ± S.E, vs control, ^aP<0.05; vs TGF-β1,^bP<0.05.