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. 2019 Jun 24;8:e46349. doi: 10.7554/eLife.46349

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Genetic reagent (M. muculus) B6;129S7-Amhr2tm3(cre)Bhr/Mmnc PMID: 12368913 RRID_MGI:3042214 Dr. Richard R Behringer, MD Anderson Cancer Center
Genetic reagent (M. muculus) C57BL/6-Tg(UBC-GFP)30Scha/J Jackson Laboratory stock #004353 Hongkui Zeng, Allen Institute for Brain Science
Genetic reagent (M. muculus) FVB/NCrl Charles River #207
Genetic reagent (Rat) Sprague Dawley Envigo
Peptide, recombinant protein LR-MIS (Pépin et al., 2013)
Recombinant DNA reagent AAV9-LRMIS (Pépin et al., 2015)
Antibody Smooth muscle alpha action (SMA) (Rabbit polyclonal) Abcam #5694 (1:300), IF
Antibody Vimentin (rabbit monoclonal) Abcam #92547 (1:300), IF
Antibody Foxa2 (rabbit polyclonal) LifeSpan Biosciences #138006 (1:500), IF
Antibody Cleaved caspase-3 (rabbit polyclonal) Cell signaling #9661S (1:200), IF; (1:500) IHC
Antibody E-cadherin (Cdh1) (rat monoclonal) Invitrogen #13–1900 (1:200), IF
Antibody Alexa flour 488 donkey anti rat IgG Invitrogen #A21208 (1:500)
Antibody Alexa flour 555 donkey anti rabbit IgG Invitrogen #A31572 (1:500)
Antibody Alexa flour 568 anti rabbit IgG Invitrogen #A10042 (1:500)
Commercial assay or kit RNA scope 2.5 HD red detection kit ACD bio #322360
Commercial assay or kit the target retrieval and protease plus reagents ACD bio #322330
Commercial assay or kit MIS commercial ELISA Beckmen #A73818
Commercial assay or kit REDExtract-N-Amp Tissue PCR Kit Sigma #SLBT8193
Other Bambi (M. muculus) (NM_026505.2) ACD bio #523071 commercial probe
Other Bmp7 (M. muculus) (NM_007557.3) ACD bio #407901 commercial probe
Other CD24a (M. muculus) (NM_009846.2) ACD bio # 432691 commercial probe
Other Cpxm2 (M. muculus) (NM_018867.5) ACD bio # 559759 commercial probe
Other Enpp2 (M. muculus) (NM_001136077.1) ACD bio # 402441 commercial probe
Other Hdac4 (M. muculus) (NM_207225.1) ACD bio # 416591 commercial probe
Other Misr2 (Amhr2) (M. muculus) (NM_144547.2) ACD bio # 489821 commercial probe
Other Misr2 (Amhr2) (Rat) (NM_030998.1) ACD bio # 517791 commercial probe
Other Misr2 (Amhr2) (Human) (NM_020547.2) ACD bio # 490241 commercial probe
Other Msx2 (M. muculus) (NM_013601.2) ACD bio # 421851 commercial probe
Other Myh 11 (M. muculus) (NM_001161775.1) ACD bio # 316101 commercial probe
Other Smad6 (M. muculus and Rat) (NM_001109002.2) ACD bio # 517781 commercial probe
Other Thbs2 (M. muculus) (NM_011581.3) ACD bio # 492681 commercial probe
Other Wfikkn2 (M. muculus) ACD bio # 531321 commercial probe
Software, algoritm R R Project for Statistical Computing https://scicrunch.org/resolver/SCR_001905
Software, algoritm BZ-X800 analysis software Keyence https://www.keyence.com/landing/microscope/lp_fluorescence.jsp
Software, algoritm GraphPad Prism, version 7 Graphpah