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. 2019 Jul 3;8:e45303. doi: 10.7554/eLife.45303

Figure 5. Requirement of the β1 propeller domain for Lrp4 regulation of adult neurogenesis.

(A) Domain structures of Lrp4 and deletion mutants. (B–C) Reduced Dcx+ cell density in Lrp4 CKO and Lrp4 CKO ∆β1, compared with control and Lrp4 CKO ∆β3 mice. (B) Representative images. Scale bar, 50 µm. (C) Stereological quantification of Dcx+ cell density. n = 3 for each group. One-way ANOVA multiple comparisons test, F (3,8)=18.69, p=0.0006; control vs Lrp4 CKO, p=0.0011; control vs Lrp4 CKO ∆β1, p=0.0012; control vs Lrp4 CKO ∆β3, p=0.7005. (D–E) Reduced BrdU+ cell density in Lrp4 CKO and Lrp4 CKO ∆β1, compared with control and Lrp4 CKO ∆β3 mice. (D) Representative images. Scale bar, 50 µm. (E) Stereological quantification. One-way ANOVA multiple comparisons test, F (3,8)=21.26, p=0.0004; control vs Lrp4 CKO, p=0.0005; control vs Lrp4 CKO ∆β1, p=0.0011; control vs Lrp4 CKO ∆β3, p=0.5538. Data are mean ± s.e.m. ns, p>0.05; **, p<0.01; ***, p<0.001.

Figure 5—source data 1. Requirement of the β1 propeller domain for Lrp4 regulation of adult neurogenesis.
elife-45303-fig5-data1.xlsx (11.9KB, xlsx)
DOI: 10.7554/eLife.45303.021

Figure 5.

Figure 5—figure supplement 1. Generation of Lrp4 CKO ∆β1 and Lrp4 CKO ∆β3 mice.

Figure 5—figure supplement 1.

(A) Transgene constructs. (B) Mouse crossing strategy. mt indicates either Lrp4-∆β1 or Lrp4-∆β3. (C–D) Genotyping of indicated mice. wt allele generated 415 bp; floxed allele generated 455 bp; LSL-∆β1 generated ~567 bp; LSL-∆β3 generated ~842 bp; and GFAP-Cre generated ~150 bp bands. (E) Expression of Lrp4-∆β1 and Lrp4-∆β3 in indicated mouse strains, n = 3 for each group. Western blotting was performed with indicated antibodies.