Figure 4.

T_reg cells and Foxp3^lo T_regP cells show different localization in the thymus. a) Top left panels, representative histocytometry dot plots of thymocytes stained with antibodies to CD25, Foxp3 and TCRβ used to denote Foxp3^lo T_regP cells (CD25⁻Foxp3⁺TCRβ⁺) and T_reg cells (CD25⁺Foxp3⁺TCRβ⁺) within stained thymic sections. Bottom left panels, representative images of Foxp3^lo T_regP cells and T_reg cells. Right panels, thymic sections stained with antibodies to TCRb and RORgt used to delineate the thymic medulla (M) and cortex (C). Distribution of CD25⁺Foxp3⁺TCRβ⁺ T_reg cells (blue) and CD25⁻Foxp3⁺TCRβ⁺ Foxp3^lo T_regP cells (orange) within the thymic medulla and cortex (right). Scale bar represents 200μm. b) Distribution of Foxp3^lo T_regP cells and T_reg cells between thymic cortex and medulla, p value determined by Fisher’s exact test. Data represents 1 experiment, n=2 mice. ***P<0.0001.