Table 2.

Sequences of Locked Nucleic Acid (LNA^™) primers and padlock probes for targeted pigmentation and internal control mRNAs. Lowercase letters in LNA primer sequences indicate the position of the locked nucleic acid modification. In the of padlock probes or detection oligonucleotides, sequence in blue is the hybridization site against targeted cDNA derived from corresponding mRNA, sequence in red indicates the hybridization site for corresponding detection oligonucleotide, and sequence in black is the extra fragment to stabilize padlock probe structure. Fluorescent dyes for detection oligonucleotides: FITC = Fluorescein isothiocyanate, Cy3 = Cyanines 3, Cy5 = Cyanines 5

Primer or Probe	Sequence
LNA Primers
LNA-primer ACTB	5’/CaCcTgAaCcTcTcATTGCCA
LNA-primer TYRP1	5’/AaGaCtTcGaAcTgCTGGGTC
LNA-primer MITF	5’/GtTtGgAtCcCaTtTTGGCAG
LNA-primer HPGDS	5’/TgCcAgGtCaGgTtTACAGGA
LNA-primer SLC45A2	5’/AgCaGcCaAtCaGtGCCACT
Padlock probes
Padlock-probe ACTB	5’/Phos/TGAACTCCCTGATGGCAGTGAATGCGAGTCCGTCTGACAGTCCCAGTGTACCCACCCTGGAGAAGAGCTA
Padlock-probe TYRP1	5'/Phos/GTGATCCTTCAGGAAACTAGTGCTGGATGATCGTCCGACAGTCCCAGTGTACCCACACAGTAGAAGGGTACA
Padlock-probe MITF	5’/Phos/CAGTGTTCCTGCCAATATCTGCTTATGTCGCCCGGGACAGTCCCAGTGTACCCACACCAGCTGGCACTTA
Padlock-probe HPGDS	5'/Phos/GACACTTCTGTCCTGCCTCAATGCACATGTTTGGCTCCGACAGTCCCAGTGTACCCACGGATGTGTGCAGTAC
Padlock-probe SLC45A2	5’/Phos/TCAGCATCCACAGTGAGCGATCTGCGAGACCGTATGACAGTCCCAGTGTACCCACAAACCGTGATGCCGTCACCATGATT
Detection oligonucleotide
Detection oligonucleotide ACTB	5’/FITC/ AAAAAACAGTGAATGCGAGTCCGTCTUUUU
Detection oligonucleotide TYPR1	5'-/Cy5/CCACGCCTAGTGCTGGATGATCGTCC
Detection oligonucleotide MITF	5’/Cy3/GTATCTGCTTATGTCGCCCG
Detection oligonucleotide HPGDS	5’/Cy5/CCTCAATGCACATGTTTGGCUCC
Detection oligonucleotide SLC45A2	5’/Cy3/AGCGATCTGCGAGACCGTAT