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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Nat Genet. 2019 May 27;51(6):1035–1043. doi: 10.1038/s41588-019-0417-8

Figure 3. Global amino acid variation mapped onto the protein crystal structure of the mature GAS Streptolysin O34 and C5a peptidase35.

Figure 3

(a) Frequency of amino acid variations within 2,083 genomes. (b) Schematic of the Streptolysin O and C5a peptidase open reading frame representing the location of amino acids within the mature enzymes (blue block). Model of the consensus sequence of the Streptolysin O (c) and C5a peptidase (d) mature enzymes. Plotted against the structure is the amino acid variation frequency within the 2,083 GAS genomes as represented in the colour gradient from 1% variable (blue) to 42% variable (red); invariant sites are coloured in light grey. Position of the top 5 most variable surface hotspots (“HS”) are annotated (as defined in Supplementary Tables 10 and 11). Active sites for each enzyme are indicated (cyan arrow).