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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Bioorg Med Chem. 2019 Jun 4;27(15):3254–3278. doi: 10.1016/j.bmc.2019.06.003

Figure 2.

Figure 2.

Assay for inhibition of the efflux of the fluorophore Hoechst 33342 (H33342) by 7 (A) or 22 (B) in E. coli ATCC 25922. All reactions were initiated by the addition of 50 mM glucose, except where indicated. H33342 was used at a concentration of 10 μM. Both compounds were used at concentrations ranging from 1/32X to ¼X MIC (1.56 to 12.5 μg/ml for 7 and 3.125 to 25 μg/ml for 22). The intrinsic MICs of 7 and 22 against E. coli ATCC 25922 are 50 and 100 μg/ml, respectively. Compound vehicle was DMSO.