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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Immunol. 2019 Jun 14;203(3):658–664. doi: 10.4049/jimmunol.1900358

Figure 5. Physiological function of Id2+ aTregs.

Figure 5.

(A) CD45.1 mice were irradiated and injected with bone marrow cells from WT or Id2 CKO mice. Four weeks after reconstitution, mice were placed on CD or HFD for 12 weeks. Bar graphs indicating the frequency of CD4+ Foxp3+ aTregs, ST2+ KLRG1+, ST2+ CCR2+ and GATA3+ gated aTregs in the indicated BMC under CD or HFD conditions. (B) Flow cytometry and bar graphs indicating the frequency of total macrophages (F4/80+), M1 (CD11c+ F4/80+), M2 (CD206+ F4/80+), IL-10+ F4/80+ macrophages and CD8+ T cells isolated from the adipose tissue of WT or Id2 CKO mice on CD. (C) Graphs indicating weight, fasting glucose, fasting insulin and blood glucose and insulin over time following GTT. Calculated area under the curve (AUC) from all mice tested by GTT. (D) Graph indicating the expression of TNFα, SAA3, RANTES, IL-6 and A20 mRNA fold change in total adipose tissue SVF versus spleen. Data are representative of two independent experiments with 2-3 mice per group (A, B and D) and one experiment with 6 mice per group (C). P values were calculate using the student’s t test or one-way ANOVA.