Figure 1:

To ensure repeated measures did not alter Y-maze performance, the mice were examined three times on a once per week schedule following the same protocol to determine if there were any chances in performance. Each mouse was exposed to the Y-maze for 10 minutes after a 15-minute acclimation period to the testing room. For each 10-minute test, spontaneous alternations were calculated and averaged for each group. All values represent the mean ± SEM. N = 7–8 per group. There was no significant difference in the from the first to third trial in either the 3 month or 12 month mice indicating that his is a stable phenotype that is amenable to repeated measures designs.