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. 2019 Jul 23;9:10636. doi: 10.1038/s41598-019-47011-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MIR-FEL irradiation and thermal processing. (A) Infrared microscopy observation after the FEL irradiation at 6.1 μm (left), optical microscope image of the fibril on gold-coated glass slide before heating (293 K), and those after heating (318 K and 363 K). White bar: 500 μm; black bar: 125 μm. The irradiated position was indicated by red dotted line, and visual fields for collecting infrared spectra were shown as nineteen yellow squares (100•100 μm each). (B) Averaged spectra of fibrils after irradiation (upper), heating at 318 K (middle), and heating at 363 K (bottom). For the heated samples, about 20 spectra were recorded using 20•20 μm apertures and averaged. (C) Conformational analysis. Labels were the same with those in Fig. 4C.