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. 2019 Jul 23;9:10684. doi: 10.1038/s41598-019-47116-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autophagy core complex proteins are increased under SMG. Western blot images for Autophagic core complex proteins (Autopahgy-Related proteins) ATG3, ATG5, ATG7, ATG12, ATG16L1 at 48 h post 3D or SMG (A) and its corresponding graph (B) representing mean ± S.D of fold change. Western blot images for autophagy regulator Beclin1 and upstream regulators of autophagy (Forkhead box O3) FOXO3, (protein kinase B) AKT, (phosphatase and tensin homolog) PTEN and housekeeping protein GAPDH at 48 hours post 3D or SMG (C). the corresponding graph of expression fold change (D). The data represented as mean ± S.D. The expression intensity was measured through densitometry of western blot images fold change was normalized against GAPDH. The experiments were repeated thrice *P < 0.05, **P < 0.005, ***P < 0.001, statistical analysis done using unpaired t-test with Welch’s correction.