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. 2019 Jul 23;9:10684. doi: 10.1038/s41598-019-47116-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nuclear YAP causes Yamanaka factor expression. Images for western blot for β-Catenin, (yes associated protein) YAP and Yamanaka factors (Octamer-binding transcription factor 4) OCT4A, (SRY-Box 2) SOX2, (Homeobox protein) Nanog and (NK2 Homeobox 5) NKx-2.5 (A) among control, 3D, SMG, Autophagy inhibition and Autophagy elevation. The protein expression represented as fold change normalized against GAPDH, compared against control (B) for proteins YAP, β-Catenin and OCT4A and bar graph of protein expression of SOX2, Nanog and NKX2.5 (C). The experiments were repeated thrice and the data represented as mean + S.D. *P < 0.05, **P < 0.005, ***P < 0.001, statistical analysis done using unpaired t test with Welch’s correction. The black bars represent control, light bars represent 3D and dark grey bars SMG. The bars with (/) lines represent AI and bars with (\) lines represent AE.