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. 2019 Jul 23;10:3293. doi: 10.1038/s41467-019-11149-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HIFα Met_n-3 is conserved in the vertebrate lineage. a, b Blue = vertebrate; orange = invertebrate. Full-length HIF1α cDNA sequences were aligned. The region corresponding to the oxygen-dependent degradation (ODD) domain was re-aligned and the top 21 alignments were concatenated. a A maximum-likelihood phylogenetic tree was constructed. Branch values indicate bootstrap support (100 bootstraps). Scale bar, 0.7 substitutions per site. b Non-synonymous (dN) and synonymous (dS) substitution rates were estimated for codons that aligned without gaps using the HyPhy software accessed via Mega7. The amino acid residue and numbering correspond to human HIF1α. c Annotated HIF1α amino acid sequences (full-length) were identified through use of BLASTP. Sequences were aligned using the MAFFT algorithm via the GUIDANCE2 webserver. The frequency of the amino acid three residues N-terminal of the primary hydroxylation site is indicated for vertebrate and invertebrate species