Figure 2.

T cell activation does not contribute to aggravated atherosclerosis in Cd47^−/− mice. WT and Cd47^−/− mice were injected once with AAV-PCSK9^DY and fed high-cholesterol diet for 10 weeks (n = 7–8). Quantification of splenic lymphocytes (A). Splenic CD4⁺ and CD8⁺ T effector cells (B) and Ki67⁺ cells (C). Levels of DCs (D), CD11b⁺SIRPα⁺ DCs (E) and CD86 mean fluorescence intensity (MFI) on DCs (F). Quantification of plaque area and lesion area % of vessel area in WT and Cd47^−/− mice given depleting anti-CD4/anti-CD8 mAbs (n = 7–9; G,H). *p < 0.05, **p < 0.01, ***p < 0.001.