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. 2019 Jul 23;9:10608. doi: 10.1038/s41598-019-46942-x

Figure 3.

Figure 3

CD47-deficiency mice display increased numbers of CD90+ IFN-γ-producing NK cells. Expression of CD90 on NK cells in spleen, liver and aorta of WT and Cd47−/− mice given anti-CD4/CD8 depleting mAb (A; n = 7–9) WT and Cd47−/− mice were injected once with AAV-PCSK9DY and fed a HCD for 8 weeks (n = 7–8). Levels and numbers of splenic NK cells (CD3−NK1.1+; B,C). Quantification of CD90+ NK cells in blood, spleen, liver and aorta (D,E). NK cell production of IFN-γ after 4 hours of Brefeldin A or phorbol 12-myristate 13-acetate, ionomycin and Brefeldin A stimulation (F,G). Comparison of IFN-γ production of CD90− and CD90+ NK cells (H). WT or Cd47−/− mice were injected with eFluor670 labeled WT NK cells. CD90 expression of transferred cells measured seven days after transfer (I). Levels of NK cells (J), and levels and numbers of CD90+ NK cells (K,L) in bone marrow of untreated WT and Cd47−/− mice. *p < 0.05, **p < 0.01, ***p < 0.001.