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. 2019 Jul 23;9:10681. doi: 10.1038/s41598-019-47128-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OS cells phosphorylation profiling affected by PAM. Phospho-kinase antibody array showing the phosphorylation/activation status of a panel of kinases in SaOS-2 cells after treatment with PAM +/− Pyr (10 mm, 1 L/min, 15 min) or H₂O₂ (400 μM) for 6 hours. (A) Representative spots and numbers listed correspond to 13/43 selected kinases. (B) Selected spots were digitized and mean pixel intensity is presented as columns (untreated) or colored markers (treatments). (C) Venn diagram illustrating the number of similar phosphorylation pattern between treatments PAM + Pyr (blue), PAM-Pyr (red) and H₂O₂ (yellow): PAM + Pyr & PAM-Pyr (2/13), PAM + Pyr & H₂O₂ (0/13), PAM-Pyr & H₂O₂ (12/13). Complete images of the phospho-kinase antibody array are presented as Fig. S2.