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. 2019 Jul 23;9:10619. doi: 10.1038/s41598-019-46831-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Circular representations of the pEM148 plasmid of E. marmotae. From the outside in (to scale): circle 1 represents genes on the positive and negative strands (scale is marked in 50 kb), circle 2 shows a plot of GC content (higher values outward), and circle 3 shows a plot of GC skew (G − C)/(G + C). The red curves indicate the region to be compared. Arrows of inset indicate predicted ORFs in both strands. Shown below gene bar are locus tags. Regions in light gray indicate homologous sequences and percentages of identity between two homologous genes at the nucleotide level. The inset depicts the comparisons of the plasmid regions of T3SS, T2SS and sfp gene cluster with the corresponding regions of pCP301 of Shigella flexneri str. 301 (NC_004851), E. coli 545 chromosome (NZ_CP018976) and sfp cluster of E. coli plasmid pCFSAN004177G_03(CP012494).